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Bcdat medium

WebNov 18, 2024 · BCDAT medium is composed of 1 mM MgSO 4, 1.84 mM KH 2 PO 4 (pH 6.5), 10 mM KNO 3, 45 μM FeSO 4, trace element solution (0.22 μM CuSO 4, 10 μM H 3 … WebSep 8, 2024 · The cellophane and cultures were then transferred onto BCDAT agar medium containing various concentrations of arginine and cultured for an additional 7 days. The pH of the BCDAT medium was 6.5, but was modified to 8.5 with KOH to prepare an increased-pH control for BCDAT medium supplemented with 3.0 mM arginine. …

BCD medium - CSH Protocols

WebFeb 16, 2007 · For immunofluorescence, tissue grown on BCDAT or BCD medium was harvested 10 and 15 d after inoculation. Immunofluorescence Microscopy. Tissue from P. patens cultured on BCDAT or BCD medium was prepared for immunofluorescent localization of mannan as described previously (McCartney et al., 2003). Briefly, tissue … WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m -2 s -1 ): dark … cravatte lana https://retlagroup.com

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WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m-2 s-1): dark cycle. To induce sporophytes, tissues were grown for 3-6 months on peat plugs at 16°C with an 8 h: 16 h, light (300mmol m-2 s-1): dark cycle. Sporangia were sterilized in 20% … WebMar 30, 2024 · BCD medium (BCDAT without ammonium tartrate) may alternatively be used to grow and observe gametophore tissues. 3. This medium can be used several … WebSep 1, 2012 · A mixture of chloronemata and caulonemata was homogenized by vortexing for 1 minute using a six-well tube and ceramic balls (KURABO, Osaka, Japan), and vegetatively propagated on a BCDAT medium agar plate for four days. Then, the tissues were treated with or without phytohormones. cravatte ascot

DNA damage triggers reprogramming of differentiated cells into …

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Bcdat medium

Constitutive auxin response in Physcomitrella reveals …

WebApr 8, 2024 · patens protonemal tissue was grown on BCDAT medium with and without β-estradiol for 2 days and total RNA was isolated using Trizol and a RNeasy mini kit … WebDec 7, 2015 · Transformants were confirmed after three rounds of selection on BCDAT medium supplemented with G418 medium (30 μg·mL −1) alternatively for 15 days each …

Bcdat medium

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WebJan 28, 2024 · GFP-mTalin transformants for cdka-dko deficiency were selected on BCDAT medium containing hygromycin (30 mg/liter) or zeocin (100 mg/liter) and further confirmed by polarotropism defects under polarized light. To observe cp-actin, a confocal laser scanning microscope was used (Zeiss LSM 510). The objective lens was a Plan … WebJul 22, 2024 · Survivors were transferred to plates containing BCDAT medium, and then the plates were vertically oriented in darkness for phenotypic screening (Fig. 1a ). Several mutants whose protonemata grew...

WebOn minimal BCD medium, protonemal growth was strongly reduced and eventually ceased after a month (Figure 2A, C and G). At this stage, colonies comprised both chloronemal and caulonemal cells and... WebOct 23, 2024 · A microdevice with protonema cells was submerged in BCDAT medium to prevent liquid evaporation from the channels; the dish was sealed with parafilm and left for 3–4 d at 25 °C under continuous ...

WebMay 20, 2016 · Chloronema-to-caulonema conversion takes place more rapidly on the BCD medium than on the BCDAT medium. 19. It is convenient to keep some BCD agar media in a 1.5 ml tube, since the tube can be heated in the heat block and the medium becomes molten quickly (100 °C, 2 min). 20. A thinner layer of the agar medium is better for clear … WebJun 1, 2024 · Finally, half of the gametophores of S0, S1 and R1 were used for total RNA extraction, and half of R1 gametophores were transferred to BCDAT medium for continued growth. Arabidopsis thaliana ( A. thaliana ) seeds, ecotype Col-0 were washed with 75% Ethanol for 5 min, 5% sodium hypochlorite for 10 min, then washed with water for 10 …

WebAll strains were cultured either as 'spot inocula' on BCD agar medium supplemented with 1 mM CaCl 2 and 5 mM ammonium tartrate (BCDAT medium) or as lawns of protonema …

WebSep 20, 2024 · The moss leaf cells were cultured in BCDAT medium. Liberalities, the information entropy of transcriptome were measured in each culture time. Figure 2: Bar charts of leaf cells transcriptome during reprogramming. The occupation rate of genes in a transcriptome was plotted in a bar chart. Heights of boxes in a bar chart indicate the … mail mediaworld servizio clienticravatte leonardWebApr 19, 2024 · P. patensprotonemal tissue was subcultured routinely at 7-day intervals on cellophane disks (AA packaging) overlaying Petri dishes (90 mm in diameter) that … mail medication incinerationWebBCDATG or BCDAT medium is used for sub-culture of P. patens protonemata. Protonemata for PEG-mediated transformation are sub-cultured on BCDATG agar … cravatte lanvinWebtoplasts per plate) containing BCDAT medium with mannitol and incubated in the light (15Wm 2) for 6d. Antibiotic-resistant plants were selected by transfer of the cellophane … cravatte leonard parisWebJun 1, 2016 · (E–H) Plants grown for one month on BCDAT medium to promote filamentous growth. (E, F) WT plant grown without auxin or with 12.5 μM NAA, respectively. (G) iaa2 … mail medionWebSep 12, 2024 · They were then moved to BCDAT plates supplemented with 50 μg/ml hygromycin by transfer of the cellophane discs. After 10-14 days, cellophane disks were transferred to BCDAT medium without antibiotic. After an additional 10-14 days, growing colonies were transferred back to BCDAT plates supplemented with hygromycin for a … cravatte da stampare per la festa del papa