WebNov 18, 2024 · BCDAT medium is composed of 1 mM MgSO 4, 1.84 mM KH 2 PO 4 (pH 6.5), 10 mM KNO 3, 45 μM FeSO 4, trace element solution (0.22 μM CuSO 4, 10 μM H 3 … WebSep 8, 2024 · The cellophane and cultures were then transferred onto BCDAT agar medium containing various concentrations of arginine and cultured for an additional 7 days. The pH of the BCDAT medium was 6.5, but was modified to 8.5 with KOH to prepare an increased-pH control for BCDAT medium supplemented with 3.0 mM arginine. …
BCD medium - CSH Protocols
WebFeb 16, 2007 · For immunofluorescence, tissue grown on BCDAT or BCD medium was harvested 10 and 15 d after inoculation. Immunofluorescence Microscopy. Tissue from P. patens cultured on BCDAT or BCD medium was prepared for immunofluorescent localization of mannan as described previously (McCartney et al., 2003). Briefly, tissue … WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m -2 s -1 ): dark … cravatte lana
Plates with 2-FA resistant foci of wild type ... - ResearchGate
WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m-2 s-1): dark cycle. To induce sporophytes, tissues were grown for 3-6 months on peat plugs at 16°C with an 8 h: 16 h, light (300mmol m-2 s-1): dark cycle. Sporangia were sterilized in 20% … WebMar 30, 2024 · BCD medium (BCDAT without ammonium tartrate) may alternatively be used to grow and observe gametophore tissues. 3. This medium can be used several … WebSep 1, 2012 · A mixture of chloronemata and caulonemata was homogenized by vortexing for 1 minute using a six-well tube and ceramic balls (KURABO, Osaka, Japan), and vegetatively propagated on a BCDAT medium agar plate for four days. Then, the tissues were treated with or without phytohormones. cravatte ascot